IMedia yeNkcubeko yeSeli yiQonga loPhuhliso oluLungisiweyo
Imidiya yenkcubeko yeseli ngumhluzi wezondlo oqulethe izondlo ezibalulekileyo kunye nezinto ezikhulayo ezifunekayo ekukhuleni kweeseli kunye nokugcinwa.Ngokuqhelekileyo iqulunqwe ngumxube olungelelanisiweyo weekhabhohayidrethi, iiproteni, iilipids, iiminerali, iivithamini, kunye nezinto ezikhula.Ijelo leendaba likwabonelela ngemeko-bume efanelekileyo ukuze iiseli zikhule kakuhle kuyo, njengepH efanelekileyo, uxinzelelo lweosmotic nobushushu.Imithombo yeendaba inokuba ne-antibiotics ukuthintela ukungcoliseka kwebhaktheriya okanye ukungunda, kunye nezinye izongezo zokuphucula ukukhula kweentlobo ezithile zeeseli.Imidiya yenkcubeko yeeseli isetyenziswa kwiintlobo ngeentlobo zophando kunye nezicelo zonyango, ezifana nobunjineli beethishu, ukufunyanwa kweziyobisi, kunye nophando lomhlaza.
I-Stem Thengisa iMedia yeNkcubeko
Imithombo yeendaba yenkcubeko yeseli ye-Stem idla ngokuba nendibaniselwano yesiseko sesiseko, esifana ne-Dulbecco's Modified Eagle Medium (DMEM) okanye i-RPMI-1640, kunye ne-serum supplement, njenge-fetal bovine serum (FBS).I-basal medium ibonelela ngezondlo eziyimfuneko kunye neevithamini, ngelixa isongezelelo se-serum songeza izinto zokukhula, ezifana ne-insulin, i-transferrin kunye ne-selenium.Ukongeza, imidiya yenkcubeko ye-stem inokuba ne-antibiotics, efana ne-penicillin, ukuthintela ukosuleleka ziibhaktheriya.Kwezinye iimeko, izongezelelo ezongezelelweyo, ezifana nezinto eziphinda ziphinde zikhule, zingongezwa kumajelo eendaba enkcubeko ukukhulisa ukukhula kwe-stem cell okanye ukwahlula.
Iseli yeStim ye-Embryonic yomntu
Iiseli ze-Embryonic stem cells (ESCs) zi-stem cells ezithathwe kubunzima beeseli zangaphakathi ze-blastocyst, i-embryo ekwinqanaba lakwangoko.Ii-ESC zabantu zibizwa ngokuba zii-hESCs.Ziyi-pluripotent, okuthetha ukuba ziyakwazi ukwahlula kuzo zonke iindidi zeeseli zomaleko omathathu entsholongwane esisiseko: i-ectoderm, i-endoderm kunye ne-mesoderm.Zisisixhobo esixabiseke kakhulu sokufunda ibhayoloji yophuhliso, kwaye ukusetyenziswa kwabo okunokubakho kumayeza okuvuselela ukunyanga izifo ezininzi kube yingqwalasela yophando oluninzi.
