1.Ukukhetha umgca weseli ochanekileyo
Xa ukhetha umgca weseli ofanelekileyo wovavanyo lwakho, nceda uqwalasele ezi nqobo zilandelayo:
a.Iintlobo: Imigca yeeseli ezingezozamntu kunye nezingezizo iiprimate zihlala zinezithintelo zokhuseleko lwebhayoloji ezimbalwa, kodwa ekugqibeleni ulinge lwakho luya kugqiba ukuba usebenzise inkcubeko yohlobo oluthile.
b.Iimpawu: Yintoni injongo yovavanyo lwakho?Umzekelo, imigca yeeseli ephuma kwisibindi kunye nezintso inokuthi ifaneleke ngakumbi kuvavanyo lwetyhefu.
c.Inyinwe okanye iyaqhubekeka: Nangona ukukhetha kumgca weseli olinganiselweyo kunokukunika iinketho ezininzi zokubonisa umsebenzi ochanekileyo, imigca yeseli eqhubekayo ilula ngokulula ukuyidibanisa nokugcina.
d.Eqhelekileyo okanye eguqulweyo: Imigca yeeseli ezitshintshileyo ngokuqhelekileyo inezinga eliphezulu lokukhula kunye nokusebenza kakuhle kwembewu, iyaqhubeka, kwaye ifuna i-serum encinci kwinkcubeko yenkcubeko, kodwa i-phenotype yabo iye yatshintsha ngokusisigxina ngokuguqulwa kofuzo.
e.Iimeko zokukhula kunye neempawu: Zithini iimfuno zakho zesantya sokukhula, ukuxinana koxinzelelo, ukusebenza kakuhle kwe-cloning kunye nokukwazi ukukhula kokunqunyanyiswa?Umzekelo, ukuvakalisa iiproteni eziphinda-phindaneyo kwizivuno eziphezulu, kunokufuneka ukhethe imigca yeeseli enamazinga okukhula okukhawulezayo kunye nokukwazi ukukhula ekumisweni.
f.Ezinye iikhrayitheriya: Ukuba usebenzisa umgca weseli oqingqiweyo, ngaba kukho isitokhwe esaneleyo esikhoyo?Ngaba umgca weseli ubonakaliswe ngokupheleleyo, okanye kufuneka uyiqinisekise ngokwakho?Ukuba usebenzisa umgca weseli ongaqhelekanga, ngaba kukho umgca weseli olinganayo onokusetyenziswa njengolawulo?Ingaba umgca weseli uzinzile?Ukuba akunjalo, kulula kangakanani ukuyilinganisa kwaye wenze isitokhwe esaneleyo somkhenkce sovavanyo lwakho?
2.Obtain imigca yeseli
Ungazakhela eyakho inkcubeko kwiiseli zokuqala, okanye ungakhetha ukuthenga iinkcubeko zeeseli ezimiselweyo kubaboneleli bezorhwebo okanye abangenzi nzuzo (okt iibhanki zeeseli).Ababoneleli abahloniphekileyo banikezela ngemigca yeeseli ezisemgangathweni eziye zavavanywa ngokucokisekileyo ukuthembeka kunye nokuqinisekisa ukuba inkcubeko ayinakho ukungcola.Sicebisa ukuba singaboleki iinkcubeko kwezinye iilabhoratri kuba zinomngcipheko omkhulu wokungcoliseka kwenkcubeko yeeseli.Nokuba yeyiphi na imvelaphi yayo, nceda uqinisekise ukuba zonke iiseli ezintsha zivavanyelwe ukungcoliseka kwe-mycoplasma ngaphambi kokuba uqale ukuyisebenzisa.
Ixesha lokuposa: Feb-01-2023