Kwinkqubo yolwakhiwo lomgca weseli, udibaniso olungacwangciswanga lubhekiselele ekufakweni okungacwangciswanga kofuzo lwangaphandle kwiloci engafanelekanga yegenome yomkhosi.Nangona kunjalo, ukudityaniswa okungacwangciswanga kunemida kunye neentsilelo, kwaye ukudityaniswa okujoliswe kuyo kutshintsha ngokuthe ngcembe ngenxa yeenzuzo zayo.Eli nqaku liza kunika inkcazo ecacileyo yokuba kutheni ukuhlanganiswa okujoliswe kuyo kuthatha indawo yokuhlanganiswa okungahleliwe kwaye kuxoxwe ngokubaluleka kwayo kulwakhiwo lweeseli.
I. Ukuba bhetyebhetye kunye nokuChaneka
Ukuhlanganiswa okujoliswe kuyo kunika ukuguquguquka okuphezulu kunye nokuchaneka xa kuthelekiswa nokuhlanganiswa okungahleliwe.Ngokukhetha iisayithi ezithile zokudityaniswa, iijini zangaphandle zinokufakwa ngokuchanekileyo kwiindawo ezifunwayo zejenome yomkhosi.Oku kuthintela utshintsho olungeyomfuneko kunye nokuphazamiseka kofuzo, okwenza ulwakhiwo lweeseli lulawuleke ngakumbi kwaye luqikeleleke.Ngokwahlukileyo, ukudityaniswa okungacwangciswanga kunokubangela ukufakela okungasebenziyo, iikopi ezininzi okanye ezingazinzanga, ezinqanda ukwenziwa ngcono kunye nokuguqulwa kwemigca yeseli.
II.Ukhuseleko kunye nozinzo
Ukuhlanganiswa okujoliswe kuko kubonelela ngokhuseleko oluphezulu kunye nokuzinza kulwakhiwo lweeseli.Ngokukhetha iisayithi zezibuko ezikhuselekileyo kunye nenye indawo egcinayo yohlanganiso, iimpembelelo ezinokuthi zibe kho kwigenome yomkhosi ziyacuthwa.Ngenxa yoko, ukufakwa kweemfuza zangaphandle akukhokelela ekubonakalisweni okungaqhelekanga okanye ukuguqulwa kofuzo kwi-host host, ukuqinisekisa ukuzinza kunye ne-biosafety ye-cell line.Ngokwahlukileyo, ukuhlanganiswa okungahleliwe kunokubangela ukuhlengahlengiswa kwemfuza okungalindelekanga, ukulahleka kwemfuza, okanye ukuziphatha okungaqhelekanga kweselula, ukunciphisa izinga lempumelelo kunye nokuzinza kokwakhiwa komgca weseli.
III.Ukulawuleka kunye nokuqikelela
Ukuhlanganiswa okujoliswe kuko kunika ukulawulwa okukhulu kunye nokuqikelelwa.Ngokulawula ngokuchanekileyo iisayithi zokudibanisa kunye nenani leemfuza zangaphandle, ukuguqulwa okuthe ngqo kofuzo kunokufezekiswa kwimigca yeeseli.Oku kunceda ukunciphisa iinguqu ezingabalulekanga kunye nokuphazamiseka kofuzo, ukwenza ukwakhiwa kwe-cell line ukulawulwa ngakumbi, ukuphindaphinda, kunye nokunyuka.Ngakolunye uhlangothi, iziphumo zokudityaniswa okungahleliwe azikwazi ukulawulwa ngokuchanekileyo, okukhokelela kwiintlobo ezahlukeneyo zamaselula kunye nokungaqiniseki, ukunciphisa ukuguqulwa okuchanekileyo kunye nophuhliso lwemisebenzi ethile.
IV.Ukusebenza kakuhle kunye neendleko-zokusebenza
Ukuhlanganiswa okujoliswe kuko kubonisa ukusebenza kakuhle kunye nokusebenza kakuhle kweendleko.Ekubeni ukuhlanganiswa okujoliswe kuyo kufaka ngokuthe ngqo kwi-loci efunwayo, kuthintela ixesha elide kunye nenkqubo enzima yokuhlola inani elikhulu lee-cell clones eziqulethe i-gene ekujoliswe kuyo.Ukongezelela, ukuhlanganiswa okujoliswe kuyo kunokunciphisa imfuno yokukhetha iingcinezelo ezifana ne-antibiotics, ngaloo ndlela inciphisa iindleko kunye nexesha elibandakanyekayo ekwakhiweni kweeseli.Ngokwahlukileyo, ukudityaniswa okungacwangciswanga kudla ngokufuna ukuhlolwa kwenani elikhulu lee-clones, kwaye kulucelomngeni ngakumbi ukukhuselwa ukuthotywa okanye ukuguqulwa kokungasebenzisi kwiijini ezithile, okukhokelela ekusebenzeni okuphantsi kunye neendleko eziphezulu.
Ukuqukumbela, ukuhlanganiswa okujoliswe kuyo ngokuthe ngcembe kutshintshela ukuhlanganiswa okungahleliweyo ekwakhiweni komgca weseli ngenxa yokuguquguquka okuphezulu, ukuchaneka, ukhuseleko, ukuzinza, ukulawulwa, ukuqikelelwa, ukusebenza kakuhle, kunye neendleko ezifanelekileyo.Ngenkqubela phambili eqhubekayo kunye nophuhliso lwethekhinoloji, ukuhlanganiswa okujoliswe kuyo kuya kwandisa ngakumbi izicelo zayo kulwakhiwo lweeseli kunye nobunjineli bemfuzo, ukubonelela ngamathuba amaninzi kunye namathuba ophando lwebhayoloji kunye nemveliso yemizi-mveliso.
Ixesha lokuposa: Jun-26-2023